Journal: Nature Cardiovascular Research

Article Title: Nuclear ATP-citrate lyase regulates chromatin-dependent activation and maintenance of the myofibroblast gene program

doi: 10.1038/s44161-024-00502-3

Figure Lengend Snippet: a , Tamoxifen-inducible, Cre-recombinase-mediated gene knockout of Acly and expression of R26-tdT in activated fibroblasts. b , Mice subjected to TAC at 0 week and started on tamoxifen chow to induce Cre-recombinase expression in activated CFs. Echocardiograms (Echo) performed every 4 weeks are indicated by arrows. Take-downs were performed at 8 weeks for interstitial fibrosis and gravimetrics. c , Western blot of ACLY knockout in Acly fl/fl , Postn iCre , R26-tdT CFs. d , Peak aortic pressure gradients from Postn iCre , R26-tdT mice and Acly fl/fl , Postn iCre , R26-tdT mice that underwent TAC. Unpaired two-sided t -test. Postn iCre , R26-tdT + TAC = 9 mice; Acly fl/fl , Postn iCre , R26-tdT + TAC = 8 mice. e , Echocardiographic data of mice followed 8 weeks after TAC. Long-axis, B-mode images. EF, ejection fraction; LVESV, LV end-systolic volume; LVEDV, LV end-diastolic volume. Two-way ANOVA with Tukey HSD. Comparisons between Postn iCre , R26-tdT mice and Acly fl/fl , Postn iCre , R26-tdT mice at the same timepoint. Comparisons between different timepoints of the same genotype. Postn iCre , R26-tdT + TAC = 10 mice at 0 week and 9 mice at 4 weeks and 8 weeks; Acly fl/fl , Postn iCre , R26-tdT + TAC = 10 mice at 0 week and 8 mice at 4 weeks and 8 weeks. f , B-mode speckle tracking calculations of LV strain rates at baseline (Base) and 8 weeks after TAC. Two-way ANOVA with Tukey HSD. Postn iCre , R26-tdT + TAC = 10 mice at baseline and 9 mice at 8 weeks; Acly fl/fl , Postn iCre , R26-tdT + TAC = 10 mice at 0 week and 8 mice at 8 weeks. g , Picrosirius red staining of matrix protein in 10 μm LV sections. Scale bars, 200 μm. h , Percentage of the picrosirius red (PSR) stain over the total area. Outliers were identified by Grubbs test with an alpha of 0.05 and indicated with ‘×’ in the graph. Two-way ANOVA with Tukey HSD. i , Gravimetric data from mice at 8 weeks after TAC. HW, heart weight; TL, tibia length. Two-way ANOVA with Tukey HSD. For h and i , Postn iCre , R26-tdT + sham = 7 mice; Acly fl/fl , Postn iCre , R26-tdT + sham = 5 mice; Postn iCre , R26-tdT + TAC = 8 mice; and Acly fl/fl , Postn iCre , R26-tdT + TAC = 9 mice. All data are depicted as mean ± s.e.m. Full-length western blots are available as source data.

Article Snippet: For temporal Acly deletion, male and female mice between the ages of 13 weeks and 15 weeks were randomized to TAC or sham cohorts and fed tamoxifen citrate chow (Envigo; 40 mg kg −1 d −1 ) starting the day following surgery and throughout the duration of the study.

Techniques: Gene Knockout, Expressing, Western Blot, Knock-Out, Staining

Journal: Radiology Case Reports

Article Title: COVID-19 mRNA vaccine-related myocarditis: A PRISMA systematic review, imaging approach and differential diagnoses

doi: 10.1016/j.radcr.2023.11.070

Figure Lengend Snippet: Summary of the demographics, MRI features treatment and outcomes of Covid-19 mRNA vaccine-related myocarditis.

Article Snippet: Williams , 46 , 34/M , 1d , mRNA-1273 vaccine (Moderna) , Mid anterolateral and inferolateral LV walls Se LGE and patchy My OE , Echo: Reduced ejection fraction (43%) , Aspirin, Colchicine, BB, ACE inhibitor , CR.

Techniques: Blocking Assay